abstract：:The stability of the structure of sperm whale metmyoglobin has been studied in various solutions, in the temperature range -8 degrees C to 100 degrees C, by scanning microcalorimetry, light absorption, circular dichroism, nuclear magnetic resonance spectroscopy and viscosimetry. It has been shown that in 10 mM-sodium ...

journal_title：Journal of molecular biology

authors： Privalov PL,Griko YuV,Venyaminov SYu,Kutyshenko VP

更新日期：1986-08-05 00:00:00

abstract：:Transcription of mouse mammary tumor virus DNA is stimulated by steroid hormones. The DNA sequences involved in this regulation are located in the viral long terminal repeat between positions -200 and -50 with respect to the transcription initiation site. In this region four, one distal and three proximal, in vitro bi...

journal_title：Journal of molecular biology

authors： Kühnel B,Buetti E,Diggelmann H

更新日期：1986-08-05 00:00:00

abstract：:To study mechanisms of nucleosome positioning, small circular plasmids were constructed, assembled into chromatin in vivo in Saccharomyces cerevisiae, and their chromatin structures were analysed with respect to positions of nucleosomes and nuclease-sensitive regions. Plasmids used include insertions of the URA3 gene ...

journal_title：Journal of molecular biology

authors： Thoma F

更新日期：1986-07-20 00:00:00

abstract：:The secondary structure of porin, maltoporin and OmpA protein reconstituted in lipid membranes is determined by Raman spectroscopy. The three proteins have similar structures consisting of 50 to 60% beta-strand, about 20% beta-turn, and less than 15% alpha-helix. Employing a method for structural prediction that accou...

journal_title：Journal of molecular biology

authors： Vogel H,Jähnig F

更新日期：1986-07-20 00:00:00

abstract：:We report on the preparation, crystallization and preliminary X-ray crystallographic study of the Fab fragments from a murine monoclonal anti-cardiotoxin antibody M gamma 2-3 directed against a cobra cardiotoxin. The Fab fragment has been crystallized from polyethylene glycol 8000 solutions in a form suitable for high...

journal_title：Journal of molecular biology

authors： Guillon V,Alzari PM,Grognet JM

更新日期：1986-06-20 00:00:00

abstract：:The pre-steady-state kinetics of GTP hydrolysis catalysed by elongation factor G and ribosomes from Escherichia coli has been investigated by the method of quenched-flow. The GTPase activities either uncoupled from or coupled to the ribosomal translocation process were characterized under various experimental conditio...

journal_title：Journal of molecular biology

authors： Robertson JM,Urbanke C,Chinali G,Wintermeyer W,Parmeggiani A

更新日期：1986-06-20 00:00:00

abstract：:Small nuclear ribonucleoproteins (snRNPs) containing U1 and U5 snRNAs from HeLa cells have been fractionated using a combination of isopycnic centrifugation in cesium chloride and ion-exchange chromatography on DEAE-Sepharose. The procedure is based on the extreme stability conferred upon snRNPs by Mg2+ enabling them ...

journal_title：Journal of molecular biology

authors： Lelay-Taha MN,Reveillaud I,Sri-Widada J,Brunel C,Jeanteur P

更新日期：1986-06-05 00:00:00

abstract：:The domain organization of the zymogen subunits of the first component of human complement C1s, C1r2 and the complex C1s-C1r2-C1s was studied by electron microscopy. In the absence of Ca2+, monomeric C1s was visualized as a dumb-bell-shaped molecule consisting of two globular domains (center-to-center distance 11 nm) ...

journal_title：Journal of molecular biology

authors： Weiss V,Fauser C,Engel J

更新日期：1986-06-05 00:00:00

abstract：:The effect of hairpin (cruciform) size on the regulation of gene expression was investigated by cloning a series of palindromic sequences into the non-essential J-F intercistronic region of the bacteriophage phi X174 ins6 genome. Genetic stability of the insert sequence and its effect on the growth efficiency of the p...

journal_title：Journal of molecular biology

authors： Müller UR,Turnage MA

更新日期：1986-05-20 00:00:00

abstract：:We have studied the effect of organic co-solvents (monohydric alcohols and formamide) on the dimer-tetramer equilibrium of human haemoglobin by measuring the dependence of oxygen affinity upon haemoglobin concentration over a 100-fold concentration range and analysing the data with a modified Monod-Wyman-Changeux mode...

journal_title：Journal of molecular biology

authors： Cupane A,Vitrano E,Cordone L

更新日期：1986-05-20 00:00:00

abstract：:The rate of ligation of DNA molecules into circular forms depends on the ring closure probability, commonly called the j-factor, which is a sensitive measure of the extent to which thermal fluctuations contribute to bending and twisting of DNA molecules in solution. We present a theoretical treatment of the cyclizatio...

journal_title：Journal of molecular biology

authors： Levene SD,Crothers DM

更新日期：1986-05-05 00:00:00

abstract：:The structure of replicating simian virus 40 (SV40) minichromosomes was studied by DNA crosslinking with trimethyl-psoralen. The procedure was used both in vitro with extracted SV40 minichromosomes as well as in vivo with SV40-infected cells. Both procedures gave essentially the same results. Mature SV40 minichromosom...

journal_title：Journal of molecular biology

authors： Sogo JM,Stahl H,Koller T,Knippers R

更新日期：1986-05-05 00:00:00

abstract：:We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 X 10(3) base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or th...

journal_title：Journal of molecular biology

authors： Restifo LL,Guild GM

更新日期：1986-04-20 00:00:00

abstract：:Monoclonal antibodies that react with defined regions of the heavy and light chains of chicken skeletal muscle myosin have been used to provide a correlation between the primary and the tertiary structures of the head. Electron microscopy of rotary shadowed antibody-myosin complexes shows that the sites for three epit...

journal_title：Journal of molecular biology

authors： Winkelmann DA,Lowey S

更新日期：1986-04-20 00:00:00

abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

journal_title：Journal of molecular biology

authors： Birkeland NK,Lindquist BH

更新日期：1986-04-05 00:00:00

abstract：:The five suppressors SUP35, SUP43, SUP44, SUP45 and SUP46, each mapping at a different chromosomal locus in the yeast Saccharomyces cerevisiae, suppress a wide range of mutations, including representatives of all three types of nonsense mutations, UAA, UAG and UGA. We have demonstrated that ribosomes from the four sup...

journal_title：Journal of molecular biology

authors： Eustice DC,Wakem LP,Wilhelm JM,Sherman F

更新日期：1986-03-20 00:00:00

abstract：:A transcriptionally active sub-set of the dispersed mouse VL30 family of proviral genetic elements was shown to be highly transmissible as a murine leukaemia virus pseudotype. Newly acquired VL30 proviruses (present at 1 to 2 copies per cell) were shown to be transcriptionally active. These data substantiate the hypot...

journal_title：Journal of molecular biology

authors： Carter AT,Norton JD,Gibson Y,Avery RJ

更新日期：1986-03-05 00:00:00

abstract：:The recA protein from Proteus mirabilis, which is homologous to the Escherichia coli protein, forms crystals in the orthorhombic space group P2(1)2(1)2(1). There are two 38,000 molecular weight subunits in the asymmetric unit and the unit cell dimensions are a = 57.5, b = 127.0 and c = 157.0 A. ...

journal_title：Journal of molecular biology

authors： Weber IT,Steitz TA

更新日期：1986-03-05 00:00:00

abstract：:Two recombinant plasmids containing, respectively, three and eight tandem repeats of a 177 base-pair (bp) element from radish nuclear DNA have been isolated. These plasmids were used as probes to investigate the organization and the copy number of this element within the genome. This sequence is present in congruent t...

journal_title：Journal of molecular biology

authors： Grellet F,Delcasso D,Panabieres F,Delseny M

更新日期：1986-02-20 00:00:00

abstract：:We describe fibre diffraction studies on the interaction of DNA with different amino acids and peptides. The B form of DNA, with ten base-pairs per turn, is always found at high levels of humidity. We suggest that this pitch is observed because the DNA molecules are maintained in a straight position. In solution, the ...

journal_title：Journal of molecular biology

authors： Campos JL,Portugal J,Subirana JA,Mayer R

更新日期：1986-02-05 00:00:00

abstract：:A Xenopus laevis complementary DNA (cDNA) library prepared from messenger RNAs extracted from embryos has been screened for actin-coding sequences. Two cDNA clones corresponding to an alpha cardiac and an alpha skeletal muscle actin mRNA have been identified and characterized. From a genomic library, we have furthermo...

journal_title：Journal of molecular biology

authors： Stutz F,Spohr G

更新日期：1986-02-05 00:00:00

abstract：:Silk fibroin with the alanyl carboxyl carbon enriched with 13C was obtained by giving a diet containing 13C-enriched alanine to the larvae of Bombyx mori and Antheraea pernyi at the fifth instar. Sericin-free fibroin fibers were prepared from cocoons, and gut was made from the liquid silk in the gland. The final 13C c...

journal_title：Journal of molecular biology

authors： Fujiwara T,Kobayashi Y,Kyogoku Y,Kataoka K

更新日期：1986-01-05 00:00:00

abstract：:Using purified sigma 55 RNA polymerase from Bacillus subtilis in an in vitro transcription system, we have shown that both promoters and terminators of Gram negative origin are recognized by this enzyme. Furthermore, when B. subtilis is transformed with a shuttle vector containing certain of these promoters, synthesis...

journal_title：Journal of molecular biology

authors： Peschke U,Beuck V,Bujard H,Gentz R,Le Grice S

更新日期：1985-12-05 00:00:00

abstract：:The central domain is an evolutionarily conservative region that is invariant in length in the A and Hc-A families of silkmoth chorion proteins. This domain shows strong sixfold periodicities for various amino acid residues, such as glycine and large non-polar residues. The periodicities and their phase relationships,...

journal_title：Journal of molecular biology

authors： Hamodrakas SJ,Etmektzoglou T,Kafatos FC

更新日期：1985-12-05 00:00:00

abstract：:The cleavage site of the T4 prohead protease in gene product 68 of bacteriophage T4 has been determined by direct protein sequencing. It is located close to the carboxy-terminal end of a predicted alpha-helix in the sequence Asn-Val-Glu-Ala between the Glu and Ala residues. Secondary structure seems to be more importa...

journal_title：Journal of molecular biology

authors： Keller B,Kellenberger E,Bickle TA,Tsugita A

更新日期：1985-12-05 00:00:00

abstract：:Transformation of different Escherichia coli strains by linear dimers of pBR322 containing different tet alleles was investigated. Linear dimers transformed wild-type strains 0.1 to 1% as efficiently as circular dimers. In contrast, linear dimers transformed recBrecCsbcA strains, where the RecE recombination system is...

journal_title：Journal of molecular biology

authors： Symington LS,Morrison P,Kolodner R

更新日期：1985-12-05 00:00:00

abstract：:Two crystal habits, one rod shaped and the other square prismatic, of the Fab fragment of a monoclonal anti-phenylalanine hydroxylase antibody have been grown using the method of vapour phase diffusion against polyethylene glycol 6000. The square prisms diffract to better than 2.8 A, belong to the space group P1 and h...

journal_title：Journal of molecular biology

authors： Guddat LW,Cowan SW,Machin KJ,Isaacs NW,Cotton RG

更新日期：1985-11-20 00:00:00

abstract：:DNA polymerase I of Escherichia coli provides an excellent model for the study of template-directed enzymatic synthesis of DNA because it is a single subunit enzyme, it can be obtained in large quantities and the three-dimensional structure of the polymerizing domain (the Klenow fragment) has recently been determined ...

journal_title：Journal of molecular biology

authors： Joyce CM,Fujii DM,Laks HS,Hughes CM,Grindley ND

更新日期：1985-11-20 00:00:00

abstract：:The structure and function of the apolipoproteins are of interest because of their central role in lipid metabolism. We report the complete primary structure of the human apo AII and CIII genes. These, like apo AI, contain three introns located at conserved positions. This may reflect their evolutionary and functional...

journal_title：Journal of molecular biology

authors： Shelley CS,Sharpe CR,Baralle FE,Shoulders CC

更新日期：1985-11-05 00:00:00

abstract：:The active sites of aspartate transcarbamoylase from Escherichia coli were titrated by measuring the decrease in the enzyme-catalyzed arsenolysis of N-carbamoyl-L-aspartate caused by the addition of the tight-binding inhibitor, N-phosphonacetyl-L-aspartate. Because the enzyme is a poor catalyst for this non-physiologi...

journal_title：Journal of molecular biology

authors： Foote J,Schachman HK

更新日期：1985-11-05 00:00:00

abstract：:The control of DNA replication initiation in the plasmid ColE1 is mediated by RNAI, a 108 nucleotide plasmid-encoded RNA that is entirely complementary to the 5'-terminal region of the replication primer RNA. RNAI acts in trans to inhibit primer maturation. Previously, we constructed a plasmid in which the ColE1 RNAI ...

journal_title：Journal of molecular biology

authors： Dooley TP,Tamm J,Polisky B

更新日期：1985-11-05 00:00:00

abstract：:Rabbit liver aldolase was purified by affinity elution on a CM52 ion exchanger. Crystals of rabbit liver aldolase suitable for X-ray diffraction experiments were grown from 45% saturated ammonium sulfate solution at 4 degrees C. The enzyme crystallizes in space group C222(1) having cell dimensions a = 377.02 A, b = 13...

journal_title：Journal of molecular biology

authors： Sygusch J,Beaudry D

更新日期：1985-11-05 00:00:00

abstract：:The binding sites for histones and high mobility group proteins (HMG) 14 and 17 have been located on DNA in the nucleosomal cores and H1/H5-containing nucleosomes. The nucleosomes were specifically associated with two molecules of the non-histone proteins HMG 14 and/or HMG 17 when followed by DNA-protein crosslinking ...

journal_title：Journal of molecular biology

authors： Shick VV,Belyavsky AV,Mirzabekov AD

更新日期：1985-09-20 00:00:00

abstract：:The RecBC enzyme of Escherichia coli promotes genetic recombination of phage or bacterial chromosomes. The purified enzyme travels through duplex DNA, unwinding and rewinding the DNA with the transient production of potentially recombinogenic single-stranded DNA. The studies reported here are aimed at understanding wh...

journal_title：Journal of molecular biology

authors： Taylor AF,Smith GR

更新日期：1985-09-20 00:00:00

abstract：:The importance of including different energy contributions in calculations of electrostatic energies in proteins is examined by calculating the intrinsic pKa values of the acidic groups of bovine pancreatic trypsin inhibitor. It appears that such calculations provide a powerful and revealing test; the relevant solvati...

journal_title：Journal of molecular biology

authors： Russell ST,Warshel A

更新日期：1985-09-20 00:00:00

abstract：:We describe new tests, of general application, for deciding whether two proteins or DNA sequences are significantly homologous, in cases where the relationship is neither evidently true nor evidently false. Ralston and Bishop's comparison of the c-myc oncogene with the adenovirus E1a protein is discussed as an example...

journal_title：Journal of molecular biology

authors： McLachlan AD,Boswell DR

更新日期：1985-09-05 00:00:00

abstract：:The result of binding the effectors ATP and CTP to aspartate transcarbamylase was studied by X-ray solution scattering. Binding of substrate analogues produces a substantial change in the solution scattering curve, allowing us to monitor the proportion of the different quaternary structure states present in solution. ...

journal_title：Journal of molecular biology

authors： Hervé G,Moody MF,Tauc P,Vachette P,Jones PT

更新日期：1985-09-05 00:00:00

abstract：:Diffraction data for alpha-chymotrypsin crystals at -10 degrees C were measured at 1.68 A resolution and refined by restrained structure-factor least-squares refinement. The two independent chymotrypsin molecules in the crystallographic asymmetric unit were refined independently. The overall structure of alpha-chymotr...

journal_title：Journal of molecular biology

authors： Tsukada H,Blow DM

更新日期：1985-08-20 00:00:00

abstract：:The essential cell division genes ftsQ, ftsA and ftsZ map in a cluster of cell envelope genes located at two minutes on the Escherichia coli genetic map and appear to constitute an atypical operon. These closely clustered genes are all transcribed in the same direction and yet each of these genes was independently clo...

journal_title：Journal of molecular biology

authors： Yi QM,Rockenbach S,Ward JE Jr,Lutkenhaus J

更新日期：1985-08-05 00:00:00

abstract：:Muconolactone isomerase from Pseudomonas putida crystallizes from sodium sulfate solution in space group P2(1) (a = 65.84 A, b = 105.70 A, c = 77.20 A, beta = 90.5 degrees) with ten 11,000 Mr subunits per asymmetric unit. The 7 A resolution crystal structure was solved by single isomorphous replacement followed by 10-...

journal_title：Journal of molecular biology

authors： Katz BA,Ollis D,Wyckoff HW

更新日期：1985-07-20 00:00:00